Biotecnología y mejoramiento vegetal by Consejo Argentino para la Información y el Desarrollo de la

By Consejo Argentino para la Información y el Desarrollo de la Biotecnología

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2002. American Journal of Botany, 89, 551-558. , McMullen M. and Weber G. 1998. ). The Plant Journal, 13, 281–289. , Pedersena S. and Bolund L. 1993. Repeated primed in situ labeling: formation and labeling of specific DNA sequences in chromosomes and nuclei. Cytogenetics and Genome Research, 63, 235-237. -Capítulo 4 replicación y reparación de ADN así como de la replicación de virus y plásmidos y la síntesis química de secuencias de nucleótidos. De esta manera, puede decirse que la tecnología del ADN recombinante esta integrada por diversas estrategias metodológicas, muchas de las cuales son adaptaciones de procesos naturales de la genética microbiana que han sido estudiados y se conocen en profundidad.

Kew Bulletin, 46, 307-316. , Zabel P. H. 1996. Highresolution physical mapping in Arabidopsis thaliana and tomato by fluorescence in situ hybridization to extended DNA fibers. The Plant Journal, 9, 421-430. A. V. 2007. A method for cellular localization of gene expression via quantitative in situ hybridization in plants. The Plant Journal, 50, 159-175. L. C. 2008. Gene expression analysis at the onset of aposporous apomixis in Paspalum notatum. Plant Molecular Biology, 67, 615-628. , Fernández A.

Sin embargo para otras, como es el caso del crotón (Codiaeum variegatum), es suficiente con el agregado de thidiazurón. El genotipo y el tipo y concentración de reguladores del crecimiento empleados están estrechamente relacionados. - antibióticos. En procesos de transformación con Agrobacterium tumefaciens es muy común el agregado de antibióticos del tipo cefotaxime; kanamicina y carbenicilina para la eliminación de la bacteria. En explantes de hoja de diferentes cultivares de Malus domestica se encontró que 250 mg L–1 de cefotaxime aumentan significativamente la regeneración de brotes mientras que 500 mg L–1 de carbenicilina promueven la formación de callo y la Biotecnología y Mejoramiento Vegetal II 31 kanamicina inhibe los procesos morfogénicos.

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